Dr Chihpin Huang
Professor National Chiao Tung University, Taiwan
Title: Bioconcentration and teratogenicity of decabromodiphenyl ether (BDE-209) to zebrafish embryo in co-existence of SiO2 nanoparticles
Biography
Biography: Dr Chihpin Huang
Abstract
This study is to investigate the influence of SiO2 nanoparticles (nSiO2) on the uptake behaviors and toxicity of decabromodiphenyl ether (BDE-209) by zebrafish embryo (Danio rerio). The zebrafish embryo toxicity tests are employed to evaluate the teratogenicity of nSiO2-associated BDE-209 on embryonic development of the zebrafish, including hatching success and malformation. BDE-209 concentrations on the chorion and dechorionated embryo were measured for investigating the uptake behaviors and bioaccumulation. Results show that a delayed hatching temporarily occurred under co-exposing of nSiO2 and BDE-209. Furthermore, malformation types, including spinal curvature (SC), pericardial edema (PE) and yolk sac edema (YSE), were found on zebrafish larvae at 120 hours post fertilization (hpf), especially for SC and PE. Besides, the segmentation stage (10-24 hpf) of the embryo was the most sensitive stage to BDE-209, especially when the chorion was removed. The results also revealed that the accumulative amount of BDE-209 on chorion (17.2±0.45 mg/g chorion) was remarkably greater than that on dechorionated embryo (0.37±0.01 mg/g embryo) at 48 hpf. Overall, abnormal development of zebrafish occured at the co-existence of BDE-209 and nSiO2. Consequently, nSiO2 could interfere in the transport of BDE-209 from the aquatic environment to organisms and enhance their ecotoxicity due to the synergy effects of BDE-209 adsorption on nSiO2 aggregates. All relevant evidences suggested that nSiO2 could facilitate the transport of BDE-209 towards zebrafish embryo and trigger the teratogenic response of zebrafish.